Biological control of Alternaria blight disease of cumin by using native Trichoderma harzianum isolates
کد مقاله : 1025-11THCBIOCONTROL (R1)
نیما خالدی *، فرشید حسنی
موسسه تحقیقات ثبت و گواهی بذر و نهال، سازمان تحقیقات، آموزش و ترویج کشاورزی، کرج، ایران
چکیده مقاله:
In this research antagonist effect of 10 isolates of native Trichoderma harzianum against the fungus A. burnsii in laboratory and greenhouse conditions were investigated. In order to investigate the control effect of native T. harzianum isolates on mycelial growth, the isolates were evaluated for competitive saprophytic ability, inhibition effects of volatile metabolites and culture filtrates on A. burnsii in a completely randomized design with four replications. Then impact of treatments of seed and soil with three most effective isolates of T. harzianum were tested on disease severity of Alternaria blight disease in greenhouse condition and seed germination percentages, seedling height, fresh and dry weight of roots and shoots were evaluated. Maximum percentage of inhibition based on competitive saprophytic ability belonged to the isolate T13 with 57.2 %, volatile metabolites belonged to the isolate T7 with 71.9 % and also effect of culture filtrates belonged to the isolate T20 with 77.4 %. The greenhouse results revealed that the seed treatment with T13, T7 and T20 isolates decreased the disease by 48.3 %, 54.6 % and 65.1 %, respectively. Also, soil treatment with T13, T7 and T20 isolates decreased the disease by 35.9 %, 42.7 % and 48.1 %, respectively. Soil treatment method compared to seed treatment method increases the growth factors. The results of this study showed that secretion of volatile and non-volatile compounds is the most important antagonistic mechanisms of T. harzianum against the fungus A. burnsii and the native isolates of this fungus have considerable potential as biocontrol agents.
کلیدواژه ها:
Alternaria blight, Biological control, Cumin, Disease severity, Trichoderma harzianum.
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