In Vitro investigation of anti-oomycete activity of silver nanoparticles biosynthesized by Aloysia citrodora plant extract in controlling Phytophthora nicotianae phytopathogen
کد مقاله : 1036-11THCBIOCONTROL (R2)
تکتم عطایی سلامی *1، غلامحسین شهیدی بنجار2، حسن دارم3
1دانشگاه باهنر کرمان
2دانشگاه شهید باهنر کرمان
چکیده مقاله:
Silver nanoparticles (AgNPs) are of significant importance due to their antimicrobial properties and simplicity of biosynthesis, low toxicity, high stability and suitability for various applications in many fields. The unique role of plants in the biosynthesis of these nanoparticles has been shown by numerous studies. The present study investigated the ability of Aloysia citrodora plant in the biosynthesis of silver nanoparticles and the inhibitory effect of biosynthesized nanoparticles on the Phytophthora nicotianae In vitro. Their physico-chemical characteristics revealed via several procedures. In order to evaluate the physico-chemical properties of AgNPs, TEM, UV-Visible spectroscopy, DLS and EDAX analyzes were performed. The color change of silver nitrate solution from pale green to brown indicated the biosynthesis of silver nanoparticles. In vitro anti-oomycete activity of AgNPs at 0.01, 0.005, 0.002, 0.001 and 0.000625 M was investigated. Biosynthesized AgNPs against the phytopathogen had the highest inhibition rate at 0.01 M and decreased at concentrations of 0.005 M, 0.002 M, and 0.001 M, respectively. In UV-Visible spectroscopy, the absorption peak of AgNPs was at 450 nm and TEM electro micrographs revealed their average particle size was 75 nm. EDAX analyses also confirmed the presence of Ag element in the biosynthesized samples. In general, the biosynthesis of nanoparticles by plants is an environmentally safe procedure. The use of AgNPs in the field of controlling plant diseases requires the application of the necessary safeties and standards to pace along sustainable agriculture. In other words, before set of standard safety rules, the use of AgNPs in control of
کلیدواژه ها:
Aloysia citrodora, green synthesis, Phytophthora nicotianae, silver nanoparticles, sustainable agriculture
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