Study the inhibiting Fusarium oxysporum f.sp. helianti (Foh) by biosynthesized silver nanoparticles with extracts of three plants: Dill (Anethum graveolens), Thyme (Thyamus vulgaris), and Lemon beebrush (Aloysia citrodora) in vitro
کد مقاله : 1045-11THCBIOCONTROL (R4)
نازیار زندیاوری *1، شقایق حقانی2
1گروه بیماری شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد علوم و تحقیقات تهران
2گروه صنایع غذایی، موسسه آموزش عالی خزر، محمود آباد، مازندران، ایران
چکیده مقاله:
Fusarium oxysporum f. sp. helianthi causes vascular wilt in sunflowers. Although fumigation with chemicals, soil solarization, and fungicide application are several methods introduced to control plant disease; they negatively affect the environment. Alternatively, nanotechnology-based compounds are being used to manage plant diseases. Silver nanoparticles have prominent fungicidal potential against pathogens and can be biologically synthesized by plants. In this investigation, silver nanoparticles were produced using extracts from dill, thyme, and lemon leaves. An equal proportion of plant extracts and 0.04M AgNO3 ionic solution were mixed separately for each plant to create the nanoparticles. The plants' ability to produce nanoparticles was evaluated based on the color change of the solution from green to dark green-brown, which was observed visually. The effects of silver nanoparticles biosynthesized by the three plants on the in vitro inhibition of Foh were evaluated. The results showed that all nanoparticles completely inhibited the pathogen at a concentration of 0.02M. However, at concentrations of 0.006, 0.003, and 0.001M, the nanoparticles biosynthesized by thyme demonstrated a more significant inhibition rate than those produced by the other plants. In all three samples, the minimum inhibitory concentration was 0.001, but no inhibition was found at a concentration of 0.007. To analyze the properties of the silver nanoparticles biosynthesized by thyme, TEM, DLS, and UV-visible spectroscopy were conducted. The absorption peak and the particle size were 460nm, and 80nm respectively. Based on this information, it can be inferred that synthesizing silver nanoparticles using plants could effectively manage Foh.
کلیدواژه ها:
Fusarium oxysporum f. sp. helianthi, silver nanoparticles, Dill (Anethum graveolens), Thyme (Thyamus vulgaris), Lemon beebrush (Aloysia citrodora), sunflower
وضعیت : چکیده برای ارائه به صورت پوستر پذیرفته شده است