Investigating the interaction of Trichoderma harzianum (Th) on increasing resistance to Fusarium oxysporum f.sp. helianti (Foh)
کد مقاله : 1054-11THCBIOCONTROL (R3)
نازیار زندیاوری *
گروه بیماری شناسی گیاهی، دانشکده کشاورزی و منابع طبیعی، دانشگاه آزاد علوم و تحقیقات تهران
چکیده مقاله:
In this investigation, the effect of the interaction of Th on the regulation of antioxidant defense mechanisms and signaling pathway genes in sunflowers was studied. For this purpose, 15-day sunflower roots were inoculated with Th suspension and then infected with Foh after ten days. The experiment involved measuring the size of chlorotic spots, the weight, and the length of roots. Then, the changes in H2O2 content, the expression of genes including pal1, and sod1, as well as the activity of antioxidants were analyzed. The enzymes LOX, and POD were studied at 4 different times: 0, 12, 24, and 36 hours after infection. The results reveal that the total chlorotic area and the average diameter of chlorotic spots decreased significantly by 71.33%, and 39.72% respectively during 98 hours after infection in the plants treated with Th compared to control plants. Furthermore, an oxidative burst involving H2O2 production was observed in plants inoculated with both pathogen and Th at 12hpi; however, pathogen inoculation actually boosted ROS production. Although in the Real-time PCR results, a significant difference was observed in the expression of marker genes between plants treated and not treated with Th after infection with Foh, the escalation and reduction in the expression of these genes were expressively different in various treatments. Also, the amount of antioxidant enzymes expanded visibly in plants treated with Th. The results, generally, showed that the treatment of plants with Th reduced the level of Foh contamination by increasing the metabolism of defense compounds and antioxidant enzymes.
کلیدواژه ها:
Fusarium oxysporum f.sp. helianti, antioxidant enzymes, defense compounds, Trichoderma harzianum, signaling pathway genes
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