Biosynthesis of copper oxide nanoparticles (CuNPs) using Trichoderma and their application to control of pistachio die-back disease
کد مقاله : 1089-11THCBIOCONTROL (R2)
محمدصادق خوش صحبت1، حسین علایی *2، فریبا فیلبندی3، امیر حسین محمدی4، رضا حیدریان5
1دانشگاه ولیعصر (عج)دانشکده کشاورزی گروه گیاهپزشکی
2گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ولی عصر عج رفسنجان، ایران
3دانشگاه ولیعصر (عج) دانشکده کشاورزی گروه گیاهپزشکی
4پژوهشکده پسته، سازمان کشاورزی
5پژوهشکده پسته ، سازمان کشاورزی
چکیده مقاله:
Dieback and pistachio trunk canker is a significant disease affecting pistachio-growing regions. Neoscytalidium dimidiatum and Botryosphaeria dothidea are considered as the most important causal agent of this disease. Trichoderma species, have emerged as an effective biocontrol agent against plant pathogens. Recently, the use of green nanoparticles have received special attention to control of plant diseases. In this research the ability of green synthesis of CuNPs using Trichoderma was investigated. The results revealed that upon the addition of copper nitrate solution to the crude extract of four selected Trichoderma isolates, spherical copper nanoparticles were formed with average diameter of less than 100 nm. in vitro test of the inhibitory effects of the Trichoderma crude extract and green CuNPs at concentrations of 5, 15, 25, 35 and 45% on the mycelial growth of pathogens was investigated. The results indicated a direct correlation between their concentration with the inhibition rate of pathogen growth. The highest inhibition rate was observed at concentrations of 35 and 45% of the sterilized bio-CuNPs in N. dimidiatum and B. dothidea with an average of 44.2 and 52.17%, respectively. The potential of bio- CuNPs in controlling pathogens was assessed in in vitro test by detached stem method using two pistachio cultivars Ahmadaghaei and Akbari. Spray of bio-CuNPs was performed at a concentration of 16 ppm, before and after artificially inoculating wounds with pathogens. The results showed the potential of bio-CuNPs in reduction of wound length for N. dimidiatum and B. dothidea with an average of 60 and 50%, respectively.
کلیدواژه ها:
Pistacia, Trichoderma, Biological control, Bioactive metabolite, CuNPs, Trunk canker
وضعیت : چکیده برای ارائه به صورت پوستر پذیرفته شده است