The effect of some bacterial and Trichoderma isolates in controlling the damping-off caused by Pythium aphanidermatum and promoting tomato growth
کد مقاله : 1091-11THCBIOCONTROL
نسیم رضایی *1، ناهید معرف‌زاده2، سعید عباسی2، روح‌اله شریفی2، مهیار شیخ‌الاسلامی3
1گروه گیاهپزشکی
2گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران
3مرکز تحقیقا ت و آموزش کشاورزی و منابع طبیعی استان کرمانشاه، بخش تحقیقات گیاه‌پزشکی
چکیده مقاله:
Damping-off caused by Pythium aphanidermatum is one of the most common diseases in tomato (Solanum lycopersicum) growing areas. Ten bacterial strains from Bacillus, Alcalgenes, Delftia, Lyisinibacillus, Stenotrophomonas and Pseudomonas genera and 12 fungal strains from Trichoderma species (T. afarasin, T. asperellum, T. atrobrunneum, T. brevicompactum, T. capillare , T. harzianum, T. lixii and T. virens) were tested against P. aphanidermatum in laboratory and greenhouse experiments. Antagonistic effects on the pathogen were observed in dual-culture test by Bacillus subtilis and all Trichoderma strains, in volatile compounds production test by Alcaligenes faecalis, T. afarasin T04 and T. asperellum and in mycoparasitism test by all Trichoderma strains. In greenhouse test on tomato plants (variety Karoon), eight bacterial and nine fungal strains significantly reduced the incidence of damping-off compared to the diseased control. Many treatments also increased root volume, root wet weight, and foliage wet weight and a few increased root length and dry weight and foliage length and dry weight in the presence of the pathogen. Many of the fungal and bacterial strains used in this research were efficient in the biocontrol of damping-off caused by P. aphanidermatum and promoting tomato growth traits. Also, the difference between laboratory and greenhouse results showed that despite the importance of laboratory studies for the preliminary screening of microbial antagonists, it is necessary to use greenhouse or field experiments to confirm their efficiency and final selection.
کلیدواژه ها:
Volatile Compounds, Dual-Culture, Biocontrol, Mycoparasitism, Growth Promoter
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