Epiphytic bacteria from various citrus species for biocontrol of the causal agents of citrus blast disease
کد مقاله : 1092-11THCBIOCONTROL (R2)
رقیه مرادی *1، فاطمه شهریاری2، نرگس فلاحی چرخابی3
1گیاهپزشکی، دانشکده کشاورزی، دانشگاه زنجان، ایران
2گزوه گیاهپزشکی، دانشکده کشاورزی، دانشگاه زنجان، ایران
3گروه گیاهپزشکی، پردیس ابوریحان، دانشگاه تهران، ایران
چکیده مقاله:
Citrus blast is one of the common diseases that cause damage and limit in the development of citrus orchards in most parts of the world. Considering the economic importance of citrus fruits and the spread of the citrus blast disease in the north of Iran, this study was aimed to identify epiphytic bacteria isolated from citrus with biocontrol capability against the causal agents of citrus blast disease. A total of 78 branch and leaf samples of different citrus cultivars were collected from northern and southern provinces of Iran. After isolation of 316 epiphyte strains on nutrient agar medium, the initial screening of bacterial strains was performed based on potato slices soft rot and hypersensitive reaction on tobacco. The antagonistic activity of the selected strains against species Pseudomonas orientalis, Pseudomonas syringae pv. syringae and Pseudomonas viridiflava was conducted by non-simultaneous cultivation method using chloroform in a completely randomized design with three replications. Based on in vitro studies, 30%, 5% and 10% strains indicated inhibition zone against P. orientalis (7.33_34mm), P. viridiflava 3.33_3.37mm and P. syringae pv. syringae (13.33_24.66mm), respectively. Six strains GL3P1, GL3P8, GLi2P1, GPk23P2, TP24Psh3 and GK4P3 were effective against P. orientalis and P. syringae pv. syringae and strains HN73Pl3, GNr22P1 and GL3P3 showed antagonistic activity against P. orientalis and P. viridiflava. Greenhouse evaluations in order to introduction of effective strains for commercial production are under consideration.
کلیدواژه ها:
Antagonist, Citrus blast, Epiphyte, Pseudomonas syringae pv. syringae
وضعیت : چکیده برای ارائه به صورت پوستر پذیرفته شده است