Insecticidal effect of zinc oxide nanoparticles with the pathogenic fungus Beauvaria bassiana against Callosobruchus maculatus
کد مقاله : 1097-11THCBIOCONTROL (R1)
شهناز شهیدی *1، راضیه حسن زاده2، الهه تمنادار2
1دانشگاه ولی عصر رفسنجان
2گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ولیعصر (عج) رفسنجان، ایران
چکیده مقاله:
Callosobruchus maculatus (F.) (Coleoptera: Chrysomelidae) is one of the most important storage pests of legume seeds. Entomopathogenic fungus Beauvaria bassiana, can be a good alternative to chemical pesticides to control of this pest. Formulation used in this assay was mixture of fungi+ZnO-nanoparticles+alginate (F-ZnO-A). The number of colony-forming units (CFU) of the bacterium was 1010 cells/cm2. To test the toxicity, 5 ml of three concentrations of 6.51×107, 4.14×107 and 2.11×107 CFU/cm2 were sprayed on the adult insects and the entire surface of the container (7 Cm diameter). This experiment was performed with four treatments including: F-ZnO-A; fungi+alginate; alginate+ZnO; fungi without formulation. Number of 15 adult insects placed in each Petri dish. The insects were reared in insectarium with 65% ±2 relative humidity and temperature of 25 ±2 °C in dark. Mortality was recorded after 24, 48, and 72 h. The results indicated that there are significant differences between the treatments. The interaction of three factors of time, concentration, and treatment was insignificant. The values of LC50 for the formulation containing ZnO nanoparticles and fungi, formulation without nanoparticles, the formulation without fungi but containing nanoparticles and fungi suspension were 2.93×107, 4.53×107, 3.81×107, and 5.73×107 CFU/cm2 respectively. The formulation containing ZnO nanoparticles and fungi treatments with LC50 2.93×107in 48 hours had the highest mortality. In general, the pesticidal properties of the B. bassiana fungi formulation with ZnO nanoparticles showed that it can be a suitable alternative to chemical pesticides against C. maculatus.
کلیدواژه ها:
Cowpea seed beetle, Biological control, Entomopathogenic fungi, Formulation
وضعیت : چکیده برای ارائه به صورت پوستر پذیرفته شده است