Bioinformatic study of hub genes responding to the nematode Meloidogyne graminicola in rice seedlings
کد مقاله : 1141-11THCBIOCONTROL (R1)
انیس دارم *، هنگامه طاهری
گروه مهندسی تولید و ژنتیک گیاهی، دانشکده کشاورزی، دانشگاه علوم کشاورزی و منابع طبیعی خوزستان، ملاثانی، ایران
چکیده مقاله:
The pathogen Meloidogyne graminicola is a root-knot nematode that leading to a reduction in grain yield, leaf yellowing, and eventual plant death. This study aims to elucidate the molecular aspects of rice seedling tolerance to M. graminicola by analyzing protein-protein interaction networks, hub genes, key pathways in response to infection using transcriptomics data.Using computational algorithms from Cyto-Hubba on the Cytoscape platform, hub genes were identified as those with the most interactions in the gene network. Gene ontology term analysis revealed that differentially expressed genes (DEGs) were primarily associated with protein folding and ATP-dependent chaperone activities. The identified hub genes were mainly linked to various families of heat shock proteins, influencing chloroplast growth, antioxidant enzyme activity, and the response to pathogen-induced tumors.Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis indicated that protein processing in the endoplasmic reticulum and RNA degradation metabolism pathways were significantly affected during M. graminicola infection. Since the expression levels of these genes were notably higher in the pathogen-resistant variety compared to the susceptible one, enhancing the expression of these genes in the susceptible variety may be a viable approach to increase resistance. Finally, promoter motif analysis of hub genes using the Tomtom tool ( and the GoMo tool ( identified regulatory factors mainly involved in plant growth, development, and responses to stresses such as oxidative stress. The findings of this study may contribute to understanding the basic mechanisms of plant response to M. graminicola infection, facilitating the development of transgenic crops resistant to this pathogen
کلیدواژه ها:
KEGG, Gene ontology, Promoter analysis, Biological network, transcriptomics, Meloidogyne graminicola
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