Determination of the role of the native Acrobeloides nematode and its associated microbiome in causing infection on greater wax moth larvae
کد مقاله : 1145-11THCBIOCONTROL (R1)
الهام سالاری *1، مجید فصیحی هرندی2، محمدعلی محمدی2
1گروه گیاهپزشکی، دانشکده تولیدات گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان
2Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran
چکیده مقاله:
Entomopathogenic nematodes (EPNs) are important biocontrol agents, accordingly, the isolation and identification of indigenous field-collected EPNs are imperative to effectively control endemic insect pests. The primary objective of this research project was to evaluate the pathogenicity and the influence of a native field-collected Acrobeloides nematode and its endosymbiotic bacteria on the infection of the last instar larvae of Galleria mellonella. This evaluation was conducted both independently and in a symbiotic relationship, using axenic and monoxenic infective juveniles (IJs) as well as extracted symbiotic bacteria. The pathogenicity and parasitic success of three extracted bacterial isolates associated with Acrobeloides nematode were assessed by injecting the last instar larvae of G. mellonella, with four different concentrations of the bacterial suspension (ranging from 1.5×103 -1.5×106 CFU/ml in Ringer’s solution). Among three extracted Acrobeloides-associated bacteria (Alcaligenes sp., Stenotrophomonas sp., and Acinetobacter sp.), Alcaligenes sp. exhibited the highest virulence against G. mellonella larvae. Pathogenicity assessment of axenic and monoxenic IJs of Acrobeloides nematode was evaluated using a range of nematode concentrations (10, 50, 100, 200 and 300 IJs per larva). It was observed that both axenic and monoxenic IJs were capable of reducing populations of G. mellonella larvae, with monoxenic IJs demonstrating significantly higher virulence. The highest mortality rate of G. mellonella larvae (99.1%) was recorded at a concentration of 300 IJ larva¹ for monoxenic IJs of Acrobeloides nematode. The results of this study will contribute to our understanding of the development of an effective formulation technology for an indigenous isolate of EPN from Iran.
کلیدواژه ها:
Biocontrol; Insect Pathology; Acrobeloides-Associated Bacteria; Axenic Nematode
وضعیت : چکیده برای ارائه به صورت پوستر پذیرفته شده است