Toxicity of indigenous Streptomyces species metabolites on a plant-sucking pest
کد مقاله : 1148-11THCBIOCONTROL
ساناز ایمانیان *1، علی مهرور2، جواد حامدی3، حسین صمدی کفیل4، ناصر عیوضیان کاری2
1مدرس مدعو گروه گیاه پزشکی دانشگاه جیرفت
2گروه گیاه پزشکی، دانشکده کشاورزی، دانشگاه شهید مدنی آذربایجان، تبریز
3گروه بیوتکنولوژی میکروبی، دانشکده زیست شناسی، دانشگاه تهران، تهران
4گروه میکروب شناسی و ویروس شناسی، دانشکده پزشکی، دانشگاه علوم پزشکی تبریز، تبریز
چکیده مقاله:
The negative impact of chemical pesticides on non-target organisms and the environment has been a worldwide concern for quite some time. In response, researchers propose the use of entomopathogenic bacteria or their derivatives, which pose fewer risks than chemical alternatives and are inspired by nature. Actinomycetota are particularly noteworthy for their ability to produce natural compounds with various biological properties, making them a valuable bacteria phylum. Among them, Streptomyces stands out as the most significant genus. In this study, we investigated the lethal effects of the fermentation extracts of two indigenous Streptomyces species on one-day-old first-instar Metopolophium dirhodum nymphs. The obtained extracts were diluted with ethyl acetate and used at a concentration of 50 mg/ml for bioassays. Twelve hours after inoculation, the mortality percent of Streptomyces pratensis and Streptomyces panaciradicis extracts were 92.30 ± 3.84 and 65.38 ± 11.54 percent respectively. Also, the amount of LT50 for these extracts was 1.78 and 3.68 h respectively. The metabolites found in S. pratensis were more potent and acted more quickly compared to the extract from S. panaciradicis. The GC-MS analysis revealed that the S. pratensis extract contained Fatty acid methyl esters and Terpenoids, both of which have been verified to have insecticidal properties. Given the significant insecticidal effect of S. pratensis on the tested pest and its environmentally friendly nature, it can be utilized as an effective control agent for rose-grain aphids.
کلیدواژه ها:
biopesticide, fermentation, Streptomyces, aphid, microbial control, GC-MC
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